2 Days Stem Cell course

1ST MODULE: LET’S GET STARTED ON BASICS OF STEM CELLS

RATIONAL, EVOLUTION & SCOPE OF STEM CELLS

Aims & objectives.

Historical review & evolution in stem cells.

PHILOSOPHY OF CONTEMPORARY STEM CELLS

Definition of stem cells.

Properties.

Types.

Embryonic stem cells .

Adult stem cells

Potency of stem cells

ORAL CAVITY STEM CELLS

Formation of oral mucosa.

Structure of oral mucosa.

Histology of oral mucous membrane.

Histology of epithelial connective tissue junction.

Development of tooth

Epithelial origin of stem cells

Dental origin of stem cells

Mesenchymal origin of stem cells

METHODS USED TO IDENTIFY AND CHARACTERIZE STEM CELLS

Label retaining cells

Stem cell markers

In vivo lineage tracing

POTENTIAL USES OF STEM CELLS

Tissue engineering to replace lost or damaged tissues

Diversity of Regenerative responses in the dentine-pulp complex

Potential involvement of stem/progenitor cells in dentine regeneration

Induced pluripotent stem cells

Approaches to implant stem cells from teeth in humans

Stem cells in oral disease

PRF

ONGOING TRIALS

In vitro

In vivo.

HANDS ON FOR PRF

What is fibrin

Fibrin and surgical additives

Clinical applications

Armamentarium

Techniques

Complex techniques using hematology cell separators

Simplified techniques with ready-to-use commercially available kits and 2-step centrifugation to concentrate platelets

Discussions