2 Days Stem Cell course
1ST MODULE: LET’S GET STARTED ON BASICS OF STEM CELLS
RATIONAL, EVOLUTION & SCOPE OF STEM CELLS
Aims & objectives.
Historical review & evolution in stem cells.
PHILOSOPHY OF CONTEMPORARY STEM CELLS
Definition of stem cells.
Embryonic stem cells .
Adult stem cells
Potency of stem cells
ORAL CAVITY STEM CELLS
Formation of oral mucosa.
Structure of oral mucosa.
Histology of oral mucous membrane.
Histology of epithelial connective tissue junction.
Development of tooth
Epithelial origin of stem cells
Dental origin of stem cells
Mesenchymal origin of stem cells
METHODS USED TO IDENTIFY AND CHARACTERIZE STEM CELLS
Label retaining cells
Stem cell markers
In vivo lineage tracing
POTENTIAL USES OF STEM CELLS
Tissue engineering to replace lost or damaged tissues
Diversity of Regenerative responses in the dentine-pulp complex
Potential involvement of stem/progenitor cells in dentine regeneration
Induced pluripotent stem cells
Approaches to implant stem cells from teeth in humans
Stem cells in oral disease
HANDS ON FOR PRF
What is fibrin
Fibrin and surgical additives
Complex techniques using hematology cell separators
Simplified techniques with ready-to-use commercially available kits and 2-step centrifugation to concentrate platelets